Rembrandt Institute of Cardiovascular Science

Program 12th Rembrandt Symposium

19-11-2021

09:00-09:30

Registration with coffee/tea

09.30-09.35

PLENARY OPENING
Presenters/day chairs: Prof. Paul Quax & Dr. Monique Jongbloed

09.35-11.00



ORAL PRESENTATIONS PLENARY SESSION 1 Chairs: Dr. Vivian de Waard & Dr. Ilze Bot

9.35-10.00

Prof. Dr. Connie Bezzina

Keynote lecture: Inherited Cardiac Disorders: From Monogenic to Polygenic

10.00-10.10

Maaike de Jong

Influenza and EBV specific CD8+ T-cells accumulate, but do not recognize antigen, in the atherosclerotic lesion

10.10-10.20

Maarten Graumans

Inflammation of perivascular adipose tissue as a predictor of the clinical course of COVID-19

10.20-10.30

Karl Harber

Irg1 Derived Itaconate Controls Anti-Inflammatory Responses In Macrophages, Dampening Foam Cell Formation In Atherosclerotic Mice

10.30-10.40

Dieuwke Luijten

Incidence and impact of venous thromboembolism and major bleeding in patients with glioblastoma

10.40-10.50

Viviana Maraviglia

HiPSC-derived three-dimensional cardiac microtissues to study the contribution of cardiomyocytes and cardiac fibroblasts in Arrhythmogenic Cardiomyopathy pathogenesis

10.50-11.00

Arnout Mieremet

Altered Aortic Endothelial Cell Morphology in Marfan Syndrome Mice is Restored by Resveratrol

11.00-11.30

COFFEE BREAK

11.30-12.30

POSTER BLITZ PARALLEL SESSION 1


Subject: Vascular Biology 1
Chairs: Dr. Janine van Gils & Dr. Aldo Grefhorst

11.30-11.35

Alwin de Jong

Co-stimulatory treatment enhances the protective role of CD4 and CD8 T cells in murine vein grafts.

11.35-11.40

Jill de Mol

B cell depletion skews cd4+ t cells towards a pro- inflammatory phenotype in aged atherosclerotic mice

11.40-11.45

Lucie Delfos

Mast cell-induced activation of immune cells in the atherosclerotic plaque


Marie Depuydt

Identification of CD4+CD28null cells in advanced human atherosclerosis

11.50-11.55

Geoffroy Goujon

Endothelial cells from genetic diverse mouse embryonic stem cells display varied responses to angiogenic cues.

11.55-12.00

Esmeralda Hemme

Bruton’s tyrosine kinase inhibition to suppress mast cell activation in atherosclerosis

12.00-12.05

Wietse In het Panhuis

Pharmacological treatment with designer cytokine and exercise mimetic IC7Fc reduces atherosclerosis development

12.05-12.10

Rosalie Kempkes

Reduced macrophage inflammatory responses following EZH2-inhibition

12.10-12.15

Veerle Kremer

Meg8 regulates tissue factor pathway inhibitor 2 (TFPI2) expression in the endothelium

12.15-12.20

Max Gronloh

Adhesion molecule heterogeneity in endothelial cells determines neutrophil transmigration hotspots and prevent vascular leakage

12.20-12.25

Sanne Lith

Nuclear Receptor Nur77 promotes resolution of inflammation in atherosclerosis

12.25-12.30

Vannessa Meyn

Trafficking of the VE-cadherin complex

11.30-12.30

POSTER BLITZ PARALLEL SESSION 2


Subject: Thrombosis and Stroke
Chairs: Dr. Erik Klok & Dr. Monique Jongbloed

11.30-11.35

Cindy de Jong

Risk and outcomes of anticoagulation-associated abnormal menstrual bleeding in female patients with venous thromboembolism in their fertile age – the TEAM-VTE study

11.35-11.40

Wieke Grievink

Cardiovascular risk factors: the effects of aging and smoking on the immune system

11.40-11.45

Eva Janssen

Good anticoagulation is associated with low risk of adverse event and all-cause mortality in patients with continuous flow left ventricular assist devices.

11.45-11.50

Yvonne Jongejan

Identification of small interfering RNAs for allele-selective silencing of murine von Willebrand factor

11.50-11.55

Fleur Kaptein

Prevalence, treatment and prognosis of tumor thrombi in renal cell carcinoma

11.55-12.00

Sebastiaan Laan

Characterization of endothelial cell function in a patient with recurrent gastrointestinal bleeding using patient-derived endothelial colony forming cells

12.00-12.05

Milou Stals

Ruling out pulmonary embolism in patients with (suspected) COVID-19 – a prospective cohort studie

12.05-12.10

Viola strijbis

Human Prothrombin Variants with Modifications in the Active Site S4 Subpocket Demonstrate Resistance to Dabigatran

12.10-12.15

Jérémy Thalgott

Thresholds of Endoglin Expression in Endothelial Cells Explains Vascular Etiology in Hereditary Hemorrhagic Telangiectasia Type 1

12.15-12.20

Kim Dzobo

Carotid endarterectomy plaques of patients with elevated levels of lipoprotein(a) demonstrate increased intraplaque angiogenesis

12.20-12.25

Ruben Methorst

Single nuclei RNA sequencing of the mouse superior cervical ganglia enables efficient tissue characterization at single cell resolution

12.25-12.30

Floris Tange

The Effect of Normalization on the Reliability of Quantitation of Tissue Perfusion Using Near Infrared Fluorescence with Indocyanine Green

11.30-12.30

POSTER BLITZ PARALLEL SESSION 3


Subject: Arrhythmias
Chairs: Dr. Twan de Vries & Dr. Carol Ann Remme

11.30-11.35

Karolina Andrzejczyk

Identification of genetic variants in families with early onset of atrial fibrillation

11.35-11.40

Pranav Bhagirath

Cardiac magnetic resonance based predictors of ventricular arrhythmia recurrence following catheter ablation for ventricular tachycardia: initial experience

11.40-11.45

Sophia Chen

Differences in cardiac fibrosis and sympathetic innervation patterns within non-ischemic cardiomyopathy patients with anteroseptal versus inferolateral ventricular arrhythmia substrates

11.45-11.50

Jaël Copier

Bradycardia in a long QT type 2 family: coinciding KCNH2 and HCN4 variants

11.50-11.55

Michiel Blok

Effect of the Type I Interferon Signaling Pathway on Human Immortalized Atrial Myocyte Structural and Electrical Differentiation

11.55-12.00

Evita Marsman

Consortin: a novel modulator of cardiac conduction

12.00-12.05

Marieke Nederend

Atrioventricular-block necessitating ventricular pacing after tricuspid valve surgery in patients with a systemic right ventricle: long term follow-up.

12.05-12.10

Michele Nicastro

Variants in POPDC2 cause an autosomal-recessive cardiac disorder comprising sinus and atrioventricular node dysfunction, and hypertrophic cardiomyopathy

12.10-12.15

Kaylin Palm

Altered Auto-phosphorylation of Rare TNNI3K Variants in Families Presenting Supraventricular Arrhythmias, Conduction Disease, and Dilated Cardiomyopathy

12.15-12.20

Caroline Pham

Candidate-based and unbiased approaches to identify the downstream targets of TNNI3K

12.20-12.25

Dominic Zimmerman

Genetic Factors Predisposing to Sudden Cardiac Arrest

12.25-12.30

Pim van Gorp

Transcriptome analysis of conditionally immortalized atrial myocytes: identification of a novel atrium-enriched protein as a regulator of sarcomerogenesis

12.30-13.30

LUNCH

13.35-15.00

ORAL PRESENTATIONS PLENARY SESSION 2
Chairs: Prof. Jaap van Buul & Prof. Marie Jose Goumans

13.35-14.00

Prof. Dr. Johan Kuiper

Keynote lecture: Single cell research to elucidate novel immune pathways in atherosclerosis

14.00-14.10

Joost Offerhaus

Elucidating novel mechanistic pathways in Brugada syndrome

14.10-14.20

Koen Prange

Taming the macrophage: novel players in inflammatory activation

14.20-14.30

Friso Rijnberg

4D flow MRI energetics in the Fontan circulation correlate with exercise capacity and MRI derived liver fibrosis

14.30-14.40

Gonzalo Sanches- Duffhues

GDF15 as a marker and mediator of vascular dysfunction in heart failure of non-ischemic origin

14.40-14.50

Thijs Sluiter

Short-term pre-operative Methionine Restriction protects from Vein Graft Disease via PPARα-dependent browning of perivascular adipose tissue

14.50-15.00

Robin van Eenige

Angiopoietin-like 4 dictates the day-night rhythm of metabolic brown adipose tissue activity

15.00-15.30

TEA BREAK

15.30-16.30

POSTER BLITZ PARALLEL SESSION 4



Subject: Vascular Biology 2
Chairs: Dr. Jeffrey Kroon & Dr. Roel Bijkerk

15.30-15.35

Bam van Steen

Transendothelial migration induces differential migration dynamics of leukocytes in tissue matrix

15.35-15.40

Rimke Postel

Il-21r blockade reduces atherosclerosis development in ldlr- /- mice

15.40-15.45

Rudmer Postma

Endothelial Cell Painting Assay; utilizing morphological changes in endothelial cells to stratify patients in complex diseases.

15.45-15.50

Rianne Schoon

Endothelial junction remodeling in leukocyte transmigration

15.50-15.55

Virginia Smit

Age-associated B cells accumulate with age and promote atherosclerosis

15.55-16.00

Kim Dzobo

Elucidating the atherogenicity of Lp(a): an unbiased lipidomics approach

16.00-16.05

Daphne van den Homberg

Post-transcriptional modifications in vasoactive microRNAs during hypoxia; N-6-methyladenosine

16.05-16.10

Suzanne van Wouw

Multi-omic profiling of lxr activation identifies slpg as a sterol-inducible lysoplasmalogenase in macrophages

16.10-16.15

Robin Verwilligen

Inhibition of Interleukin-4 Induced Gene 1 (IL4i1) stimulates a pro-inflammatory immune environment

16.15-16.20

Tamar Woudenberg

Endothelial integrity in intracranial atherosclerosis; the role of tight junction proteins and pro-atherogenic microRNAs in lesion development.

16.20-16.25

Lichen Zhang

Cold-Inducible RNA-Binding Protein, but not its antisense lncRNA, is a direct negative regulator of angiogenesis in vitro and in vivo via regulation of 14q32 angiomiRs microRNA- 329-3p and microRNA-495-3p

15.30-16.30

POSTER BLITZ PARALLEL SESSION 5


Subject: Metabolic Disease
Chairs: Dr. Amanda Foks & Dr. Sander Kooijman

15.30-15.35

Anastasiya Burdzina

Unravelling Mitochondrial Dysfunction in Hypertrophic Cardiomyopathy

15.35-15.40

Jayron Habine

The protective role of FHL2-deficiency in glucose metabolism.

15.40-15.45

Cong Liu

Fibroblast growth factor 21 potently reduces atherosclerosis and NASH development

15.45-15.50

Amber Meurs

Genome-wide CRISPR-Cas9 screen to identify new proteins involved in intracellular cholesterol transport

15.50-15.55

Robin van Helden

HiPSC-derived cardiomyocytes from patients with infantile mitochondrial cardiomyopathy show diminished mitochondrial capacity and disrupted calcium homeostasis

15.55-16.00

Sanne Verberk

Macrophage ATP citrate lyase deficiency alters in vitro and in vivo inflammatory outcomes: A promising new target

16.00-16.05

Carmen Yap

The antibiotic doxycycline changes aortic smooth muscle cell metabolism and phenotype

16.05-16.10

Zhixiong Ying

Mirabegron activates thermogenic tissues to increase atherosclerosis only in absence of a functional hepatic ApoE- LDLR pathway for lipoprotein clearance

16.10-16.15

Novella Nicese

Sodium butyrate increases monolayer resistance and mitochondrial mass, while decreasing proliferation in glomerular endothelial cells

16.15-16.20

Katie Hanford

The duality of endothelial fatty acid oxidation in atherosclerosis

16.20-16.25

Merel Peletier

Using antibodies targeting oxidative stress epitopes to investigate and target ischemia reperfusion injury in a 2D and 3D heart on a chip model

16.25-16.30

Yiheng Zhang

PRMT4 inhibitor TP-064 impacts both inflammatory and metabolic processes without changing atherosclerosis susceptibility of male apolipoprotein E knockout mice

15.30-16.30

POSTER BLITZ PARALLEL SESSION 6


Subject: Structural Heart Disease
Chairs: Dr. Frances de Man & Dr. Anke Smits

15.30-15.35

Paul Roos

Portal vein flow and pulsatility under different breath-hold conditions

15.35-15.40

Tamara Borsboom

Vascular Outflow Tract Development: A New Source of Smooth Muscle Cells in the Ascending Aorta

15.40-15.45

Marieke Nederend

Sacubitril/valsartan in the treatment of systemic right ventricular failure: follow-up.

15.45-15.50

Julius Heemelaar

Sailing in the mist: cardiotoxicity monitoring in adolescent and young adult osteosarcoma patients

15.50-15.55

Tom Streef

Single-cell RNA sequencing of human fetal epicardium reveals novel markers and regulators of EMT

15.55-16.00

Vincent Sier

Real-time Visualization of Vascular Remodeling by Ultra-High Frequency Ultrasound Imaging.

16.00-16.05

Diederick Verheijen

The potential role of IVUS in frequently encountered anomalous aortic origin of coronary arteries.

16.05-16.10

Fleur Zwanenburg

Fetal aortic stenosis: strong association between myocardial function on fetal ultrasound and post-mortem histology

16.10-16.15

Katerina Apelt

Functional ultrafast Doppler ultrasound imaging is a novel non-invasive and robust technique to detect and monitor early renal microvasculature defects in vivo

16.15-16.20

Paul Roos

Noninvasive MRI- and CFD-based left ventricular pressure- volume loops: Initial feasibility and Scan-Rescan reproducibility study

16.20-16.25

Yang Ge

Acute myocardial infarction induces dynamic neuronal remodelling in superior cervical ganglia

16.25-16.30

Joe Juffermans

Reproducibility of potential hemodynamic sources of aortic remodeling in patients with aneurysms of the ascending aorta and healthy volunteers

16.30-17.00

REMBRANDT PROJECT REPORTS PLENARY SESSION 3

Chairs: Prof. Patrick Rensen & Prof. Carlie de Vries

16.30-16.45

Drs. Hayat Suleiman

The role of circular RNAs in phenotypic switching of vascular smooth muscle cells

14.00-14.10

Drs. Pablo Montañés- Agudo

The RNA-binding protein QKI governs a muscle-specific alternative splicing program that shapes the contractile function of cardiomyocytes

14.10-14.20

AWARDS PLENARY SESSION 4
Presenter: Prof. Dr. Paul Quax and Dr. Monique Jongbloed

17.00-17.05

17.05-17.10

17.10-17.15

17.15-17.20

Best oral presentation

Best poster presentation Poster Blitz session 1 and 2

Best poster presentation Poster Blitz session 3 and 4

Best poster presentation Poster Blitz session 5 and 6

17.20-17.30

HeArt competition - and the winner is ....
Presenters: Dr. Carol Ann Remme & Prof. Dr. Anton Jan van Zonneveld Judges: @@

17.30-17.35

REMBRANDT GRANT 2021 AWARD

Presenter: Prof. Dr. Paul Quax

17.35-17.40

CLOSING REMARKS

Presenter: Prof. Dr. Paul Quax

17.40-18.30

Drinks